it has been proposed that organic nitrates such as glyceryl trinitrate (gtn), used in the treatment of cardiovascular diseases, act by producing nitric oxide (no). however, the biochemical pathway for no formation from gtn is not well understood. in the present study, we showed that nitrate formation from gtn, by isolated rat hepatocytes, was inhibited about 50% when cellular glutathione was de...

In this research, we investigated the cytotoxic mechanisms of Cochlodinium polykrikoidescell lysate on isolated rat liver hepatocytes.This micro algae is responsible for a severe and widespread harmful algal bloom in the Persian Gulf and Gulf of Oman (2008-2009). Isolated hepatocytes were obtained by collagenase perfusion of Sprague-Dawley rat liver.According to our results, incubation of algal...

In this research, we investigated the cytotoxic mechanisms of Cochlodinium polykrikoidescell lysate on isolated rat liver hepatocytes.This micro algae is responsible for a severe and widespread harmful algal bloom in the Persian Gulf and Gulf of Oman (2008-2009). Isolated hepatocytes were obtained by collagenase perfusion of Sprague-Dawley rat liver.According to our results, incubation of algal...

in this research, we investigated the cytotoxic mechanisms of cochlodinium polykrikoidescell lysate on isolated rat liver hepatocytes.this micro algae is responsible for a severe and widespread harmful algal bloom in the persian gulf and gulf of oman (2008-2009). isolated hepatocytes were obtained by collagenase perfusion of sprague-dawley rat liver.according to our results, incubation of algal...

Isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µM) to assess the changes in reactive oxygen species (ROS) and lipid peroxidation leading to apoptotic hepatocyte cell death. The viability of hepatocytes was declined depending on the iron concentration. One hour incubation of the cells with 100 µM iron resulted in decreased...

Isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µM) to assess the changes in reactive oxygen species (ROS) and lipid peroxidation leading to apoptotic hepatocyte cell death. The viability of hepatocytes was declined depending on the iron concentration. One hour incubation of the cells with 100 µM iron resulted in decreased...

Introduction: Severe industrial diseases result from the hepatic accumulation of mercury, cadmium or chromium in humans and on the other hand cadmium and dichromate and mercuric salts may induce lung or kidney cancer. Acute or chronic CdCl2, HgCl2 or dichromate administration induces hepatic and nephrotoxicity in rodents. Oxidative stress is often cited as a possible cause of metal induced cell...

isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µm) to assess the changes in reactive oxygen species (ros) and lipid peroxidation leading to apoptotic hepatocyte cell death. the viability of hepatocytes was declined depending on the iron concentration. one hour incubation of the cells with 100 µm iron resulted in decreased...

"Hepatocyte hybridomas" have been isolated after fusion of adult hepatocytes and alpha-fetoprotein (AFP)-producing mouse hepatoma cells. The yield of viable hybrid clones was low but could be increased by culturing the cells in the presence of insulin. On the basis of their chromosome constitution, the hybrids were classified into two groups characterized by either a single or a double set of m...